Institutional Repository
Thesis Issued 2026-09-07 EN

Extraction and utilization of pectin from selected fruit wastes

Author: Rokeya Begum

Abstract

This research was concerned with the screening of fruit waste as a source of pectin, isolation, physicochemical characterization, functional properties and applicability of the extracted pectin. The process of pectin extraction from jackfruit waste was standardized by evaluating three different techniques (ammonium oxalate, acid, sodium hexametaphosphate), three different conditions (pH, temperature and time) and four different drying techniques (freeze drying, spray drying, vacuum drying and oven drying) to assess its potentiality as an alternative source of commercial pectin. Among the extraction methods, the highest yield of pectin (15.17%) was obtained by sodium hexametaphosphate/HCI method containing higher amount of moisture and ash. Ammonium oxalate and sulfuric acid extracted pectin showed similar rheological and structural properties to commercial pectin though acid hydrolysis produces significantly lower yield. Pectin recovery by acid hydrolysis was further improved by optimization of extraction conditions by response surface methodology. The optimum extraction conditions of pH, temperature and time were found 1.5, 90 °C and 82 minutes respectively. At the optimum condition yield, galacturonic acid (GalA) and degree of esterification (DE) of extracted jackfruit waste pectin were 10.57, 67.0 and 66.17 respectively. Experimental data of yield, GalA and DE agreed well with predicted value. Drying methods have insignificant effect on the chemical composition but affected significantly the functional properties of jackfruit waste pectin. The gelation of jackfruit waste pectin was investigated and compared with analytical and commercial grade pectins. Jackfruit waste pectin was found high in protein content (3.87-4.19%), ash content (4.95-5.87%) and DE (63-78.33%) and low in molecular weight (38873- 39976 Da) than commercial and analytical grade pectin, respectively. Jackfruit waste pectin (JWP) form weak gel at high temperature. Varying the different parameters of gelation conditions such as sucrose concentration, pH and pectin concentration have influenced the gelation properties of pectin. JWP was investigated as an ingredient for preparing a drying aid for spray drying of papaya juice. Pectin addition in liquid feed significantly (p<0.01) increased powder particle size, moisture content, redness and lycopene but powder yield, density and lightness were reduced significantly (p<0.01). Papaya powder was compressed to check the compressible behavior. Maltodextrin added papaya (MP) powder tablet was found more porous and less resistant to compress than pectin added papaya (PP) powder tablet. The dissolution rate of MP was faster than PP tablet which influenced by their compaction behavior, tensile strength and powder solubility. Jackfruit waste could be potential source of pectin which could fulfill the field of food and fermaceuticals.