Abstract
Investigations were conducted at Dipta Orchids Ltd. Fulbaria, Mymensingh, during the
period from March, 2012 to December, 2015 to study clonal propagation, vegetative and
reproductive growth of Dendrobium White 5N (hybrid orchid) affected by different ratio and
doses of N:P:K formulation. Growth, development and flowering were also studied under
different shade levels, manipulated day and night temperature, plant growth regulator
(6-Bengile amino purines) and different humidity levels. Finally vase live of flowers was
investigated under different chemical mixture. The experiments were conducted to develop
protocol for commercial production of Dendrobium White 5N. Among the doses, interval
and ratios of N:P:K formulations 6.0 gL¹ of N:P:K at 30:20:10 solution applied at 3 days
interval as spray were the best for higher number of clone production mother stem and also
for growth of clones. The vegetative growth of saplings most of the vegetative growth
parameters of saplins were supported by 10.0 gL-¹ of N:P:K at 20:20:20 formulation sprayed
at 7 days intervals. Reproductive growth like inflorescence length, inflorescence diameters,
number of inflorescence plant¹ and the number of flower inflorescence ¹ were found the best
with N:P:K at 05:46:23 formulation sprayed at 3 days intervals at 10.0 gL1. The effect of
different temperature levels (Day and Night) was affected quality of flowers. Maximum
flowers were obtained in day and night temperature in 26.8/18.5:23.0/10.0:28.8/11.8°C at
Dec:Jan:Feb, compared to others treatments. Among the four levels of humidity created by 1
time spray, 2 time spray and 3 times sprayday¹ with a non spray control the best result was
revealed at 3 time spray which was similar to 2 time spray per day. Reducing of sun light
intensity by black shade net was affected growth and development of Dendrobium White 5N.
Vegetative growth of saplings was significantly enhanced by 75% shading but flowering
growth was the best with 25% shading. Different concentration of BAP viz. 0.0.0.5, 1.0, 1.5
and 2.0 mgL¹ applied as spray supported growth and development differently. Among the
concentrations 1.5 mgL¹ of BAP significantly enhanced the growth of shoots, roots, length
of inflorescence and number of flowers inflorescence after 135 DAP. Vase lives of flowers
were studied under the treatments of mixed solution of sugar, silver nitrate and citric acid.
Among the 17 treatments, sugar:silvernitrate:citric acid = 75 g:750 mg:150 mg mixed water
solution was found the best in supporting vase lives after 27 days setting. As a packing
material during transportation porous poly bag was the best over newspaper, wrapping paper
non-porous poly bag and open control for higher vase life of flower and theirefore, to be
recommended.