Institutional Repository
Thesis Issued 2026-09-06 EN

Breeding for developing drought tolerant rice genotypes

Author: Mohammad Ali

Abstract

Experiments were performed to assess drought related traits and genotypes under drought condition. Data on visual leaf rolling and drying at vegetative and reproductive stages of 50 rice genotypes were scored following standard evaluation system (SES) grades and identified 7 genotypes viz., RM-40/C-1-1-5, R-3027, W-60, Binadhan-14, Y-1281, THDB and BRRIdhan56 as drought tolerant. Most of the studied morphophysiological and yield attributing traits showed lower comparative reduction (%) under drought stress in tolerant genotypes. Of these traits, panicle length and grain yield showed high heritability, whereas high PCV, GCV and genetic advance were recorded for plant height and rolled leaf (%). PCA bi-plot separated genotypes in 2 distinct groups with extreme distances as drought susceptible and tolerant groups. From the tolerant group (RM-40/C-1-1-5, Binadhan-14, R-3027, W-60 and BRRIdhan56) and susceptible check Binadhan-7 was selected for further evaluations. Of the selected genotypes, RM40/C-1-1-5, R-3027 and W-60 produced lower reactive oxygen species (ROS, O2-and H2O2 than susceptible Binadhan-7 under drought stress. Increased ROS accumulation observed in the tolerant genotypes but their many folds up-regulation was recorded in susceptible check Binadhan-7. Tolerant genotypes except Binadhan-14 showed higher antioxidant activities. Genotypes RM-40/C-1-1-5, W-60 and R-3027 showed higher proline, chlorophyll, photosynthetic rate and RWC; whereas RM-40/C-1-1-5, R-3027 and BRRI dhan56 showed lower comparative reduction (%) in root and shoot lengths under stress than Binadhan-7. Anatomical feature showed larger vascular bundles and out growths in the tolerant genotypes. Finally, field performance of 6 selected genotypes was assessed at three drought prone locations in Bangladesh. Genotypes showed significant location effects and Magura appeared as the most suitable among 3 locations. Susceptible genotype Binadhan-7 showed drastic reduction (%) yield and yield components compared to tolerant genotypes under drought stress. Genotypes RM-40/C-1-1-5, R-3027 and W-60 were predicted as drought tolerant genotypes with comparative higher yield, and could be released immediately or source of plant breeding applications.