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Thesis Issued 2026-09-08 EN

Performance of Cassava (Manihot esculenta Crantz) Germplasm Based on Morphology, Nutritional Composition and Yield

Author: Md. Golam Mostafa

Abstract

Tubers of cassava are used as staple food, feed, and raw material in industries. Field and laboratory experiments were conducted during 2009–‘2012 under subtropical condition (24°75' N 90°50' E) to characterise cassava accessions based on important morphological characters, nutritional composition, and hydrogen cyanide (HCN) content and its elimination, growth and yield. Results across field and laboratory experiments showed significant genetic variation in canopy characters, proximate composition (crude protein, crude fat, crude fibre and ash), starch and HCN contents, and tuber yield. Eleven cassava accessions were screened based on morphological descriptors, proximate compositions of leaf and tuber (root), biomass of root and shoot. Stem scar, petiole colour, leaf-lobe character and external colour of tuber contributed to most of the variations for identification of accessions. Based on tuber and starch yields, and protein and HCN contents two accessions (Coc-A1 and Kha-A2) were selected for detail agronomic studies with the former had higher yield and protein but lower HCN content. A number of cultural practices were employed: number of shoots allowed (one vs two) from cutting, two positions of mainstem cutting (proximal vs distal) placement, three depths of planting (6, 12 and 18 cm), three methods of planting (horizontal, lateral and vertical), and six dates of planting (Jan, Feb, Mar, Apr, Nov and Dec) were used in the above two selected accessions. Results revealed that single shoot allowed from proximal region of the mainstem cutting with 12 cm depth of planting and horizontal method of cutting placement in the soil using Nov or Feb date of planting produced higher starch, tuber and biomass yields with the magnitude being greater in the Coc-A1 than in the Kha-A2. In cassava, HCN is naturally produced from plant parts during postharvest processing like chopping, boiling, and drying. Elimination of HCN by processing techniques viz., effect of boiling (100°C) and drying (50°C) on HCN removal was investigated. Irrespective of accessions, it appeared that fresh plant parts contained a high quantity of HCN (133.30 –531.50 mg kg™ fresh tissue) which was deadly harmful (lethal dose for human > 50 mg HCN 50 kg¹ body wt); and boiling and drying removed 99% HCN i.e. to a safe level. Starch and tuber yields were also investigated 2, 4, 6, 8, 10, 12 months after planting (MAP) in the two accessions, Coc-A1 and Kha-A2.It was further observed that higher starch (Ave 28.36% FW) and tuber yields (Ave 45.77 t ha¹ FW) were observed during 8 to 10 MAPs with the magnitude being again greater in the former accession. Results concluded that cassava accessions could be identified by morphological descriptors, growth, nutritional composition and yield. HCN produced naturally in cassava plants during postharvest operation could be removed to a safe level by processing. Optimal protocols for realisation of maximum biomass and yield were also obtained.