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Thesis Issued 2026-09-09 EN

The Echinochloa Crusgalli Complex in The United States

Author: Md. Arshad Ali

Abstract

An investigation was made of the taxonomic relationships of Echinochloa crusea111 and the three closely related species, E. muri— cata, crus—pavonis and E. paludigena as these taxa occur In the United States. The investigation involved studies of rx: rphologlcal, anatomical, cytological and reproductive characteristics. Studies of gross morphology involving population analysis and herbarium and field specimens provided the most reliable basis for delimitation of taxa. Characters found to be of most significance were, anther length, spikelet size, the shape and character of the tip of lemma of the fertile floret, spikelet hair length, caryopsis width—length ratio and embryo—caryopsis length ratio. Other supplementary differentiat— ing characters selected were, the nature of panicle, the presence or absence of pustulate based setae on nodes of panicle axis and branches, awn characters, the presence or absence of a palea in the lower floret, and the presence or absence of stamens In the lower floret. On the basis of these characters, seven taxa were recognized fron the complex, namely, E. muricata var. murlcata, E. muricata var. micro— stachE, E. paludigena, crus—pavonis, crusgalli var. crusga111, crusgalli var. frumentacea and E. var. Microscopic studies of culm and leaf structure of the several taxa revealed no striking differences between the taxa of the com— plex. The internal s tructure of the leaf is typically panicoid, Vith vascular bundles enclosed by a single sheath and chlorenchyma cells Of the mesophyll somewhat radiating out from the bundle sheath. Bicellular microhairs in the leaf epidermis were found to be typi.— cally panicoid and present jn varying frequencies in different taxa. Microhairs of the membranous palea of the Xos*er floret were found to be clavate or even somewhat saccate and more of the chloridoid type than Of the typical panicoid type. Three— or four—celled microhairs were not infrequent in the palea epideruiis or E. muricata var. microstachy_a. In the epidermis of most of the taxa studied, papillae were common on thc long cells. Macrohairs were observed only in crusgalli var. crusgalli. Prickle hairs were common in some forms of muricata var. muricata and crusgalli var. zelayensis. The form Of silica cells varied from saddle shaped, dumbbell shaped to nodular type. Cytological studies Of five out of the seven taxa confirmed the previous report of two levels of ploidy in the complex, the tetra— = 36, and the hexaploid 2!! = 54. ploid , 2L Some positive correlation was observed between ploidy level and pollen size. Studies of mega— sporogenesls and embryo sac development showed the development of characteristic 8—nuc1eate embryo sac and normal sexual reproduction in five taxa investigated. The characterization of taxa and determination of their distri— bution patterns were based in part- on the examination of over 6,000 herbarium specimens . IVO of the four species of the complex have vide distribution in the United States. One, Echinochloa muricata, is believed to be a native species in North America and the other, crusgalli, is generally considered to be adventive. Echinochloa muricata var. muricata is restricted to the eastern half of the country. It ranges from New Hampshire and Michigan, south to Okla— homa and eastern Texas tc Florida. Echinochloa muricata var. microstachyg has wide distribution throughout the United States but is most common in the north central, central and south central States. Echinochloa crusgalli var. crusgalli is present throughout the United States but is most common in the eastern half of the country and on the west coast. Echinochloa crusgalli var. frumenta— cea, recorded mainly from northeastern local Ities, is Only found as an escape from cultivation. Echinochloa Crusgalii var. zelayensis mainly Is localized In Texas and adjacent States. Echinochloa crus— pyvonis has only been reported from California and E. paludigena is known in the United States only from southern Florida